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HH
HH
規格:
價格:
編號:B164608
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HH
商品貨號
B164608
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
T lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
cutaneous T cell lymphoma
Age
61 years adult
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis.
Clinical Data
61 years adult
The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis.
Caucasian
male
Antigen Expression
CD2; Homo sapiens, expressed
CD3; Homo sapiens, expressed
CD4; Homo sapiens, expressed
CD8; Homo sapiens; negative
CD25; Homo sapiens; negative
CD30; Homo sapiens, expressed
HLA-DR; Homo sapiens, expressed
Receptor Expression
interleukin 2 (IL-2) beta chain, expressed
HeLa Markers
N
Comments
Although HH cells lack the alpha chain of the IL-2 receptor (IL-2R alpha), they do possess a functional p70 beta chain (IL-2R beta) of the IL-2 receptor complex.
A culture submitted to the ATCC in June of 1992 was found to be contaminated with mycoplasma, and was cured by treatment with Ciprofloxacin.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12
D5S818: 11,13
D7S820: 10
THO1: 9
TPOX: 8,11
vWA: 15,16
Name of Depositor
G Starkebaum
Deposited As
Homo sapiens
References

Starkebaum G, et al. Establishment of an IL-2 independent, human T-cell line possessing only the p70 IL-2 receptor. Int. J. Cancer 49: 246-253, 1991. PubMed: 1879969

HH is a mature T cell line derived in 1986 from peripheral blood of a patient with aggressive cutaneous T cell leukemia/lymphoma. The line was established from blood taken after extensive chemotherapy.

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