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hFOB 1.19
hFOB 1.19
規格:
價格:
編號:B164604
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
hFOB 1.19
商品貨號
B164604
Organism
Homo sapiens, human
Tissue
bone
培養基
Cell Type
osteoblast; SV40 large T antigen transfected
Product Format
frozen
Culture Properties
adherent
Biosafety Level
2 [Cells contain SV40 viral sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
fetus
Applications
The cells provide a homogenous, rapidly proliferating model system for studying normal human osteoblast differentiation, osteoblast physiology, and hormonal, growth factor, and other cytokine effects on osteoblast function and differentiation.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype
diploid, 43%; tetraploid, 57%
Derivation
This line was established by transfection of limb tissue obtained from a spontaneous miscarriage with the temperature sensitive expression vector pUCSVtsA58 and the neomycin resistance expression vector pSV2-neo. Clones were selected in the presence of 0.6 mg/mL G418.
Antigen Expression
SV40 T antigen
Genes Expressed
alkaline phosphatase
Cellular Products
alkaline phosphatase
Comments
Cells grown at a permissive temperature of 33.5°C exhibit rapid cell division (Doubling time of 36 hrs), whereas little cell division occurs at a restrictive temperature of 39.5°C (Doubling time of 96 hrs).
The cells have the ability to differentiate into mature osteoblasts expressing the normal osteoblast phenotype. At the restrictive temperatures, cell division is slowed, differentiation increases, and a more mature osteoblast phenotype is produced.
Complete Growth Medium
The base medium for this cell line is a 1:1 mixture of Ham's F12 Medium Dulbecco's Modified Eagle's Medium,with 2.5 mM L-glutamine (without phenol red). To make the complete growth medium, add the following components to the base medium: 0.3 mg/ml G418; fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 34°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 34°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium, 72%; additional fetal bovine serum, 20%; DMSO, 8%
Culture Conditions
Temperature: 34°C
Temperature Effects
Permissive temperature: 33.5°C
STR Profile
Amelogenin: X
CSF1PO: 10,13
D13S317: 11,12
D16S539: 9,13
D5S818: 11,12
D7S820: 8,10
THO1: 7,9.3
TPOX: 11
vWA: 16,18
Population Doubling Time
36 hours at permissive temp of 33.5°C
Name of Depositor
SA Harris, TC Spelsberg
Deposited As
human
U.S. Patent Number
References

Harris SA, Spelsberg TC. Immortalized human fetal osteoblastic cells. US Patent 5,681,701 dated Oct 28 1997

Harris SA, et al. Development and characterization of a conditionally immortalized human fetal osteoblastic cell line. J. Bone Miner. Res. 10: 178-186, 1995. PubMed: 7754797

Jacobs CR, et al. Differential effect of steady versus oscillating flow on bone cells. J. Biomech. 31: 969-976, 1998. PubMed: 9880053

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