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HEPM

規格:

價格:

編號:B164598

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

HEPM

商品貨號

B164598

Organism

Homo sapiens, human

Tissue

palatal mesenchyme

培養基

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

fetus

Gender

female

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

This is a human diploid cell line with the 46,XX karyotype. The modal chromosome number was 46, occurring in 76% of cells. The rate of cells with polyploidies was 4.2%. No consistent chromosome aberrations were detected.

Derivation

HEPM was derived from mesenchymal cells from the human embryo by M Macy.

Clinical Data

female

fetus

Receptor Expression

epidermal growth factor (EGF)

Comments

The cells are highly responsive to epidermal growth factor (EGF).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 10,11

D13S317: 8,12

D16S539: 11,12

D5S818: 11,13

D7S820: 8,10

THO1: 6,9.3

TPOX: 8,11

vWA: 17,18

Name of Depositor

M Macy

Deposited As

Homo sapiens

Year of Origin

July, 1979

References

Yoneda T, Pratt RM. Mesenchymal cells from the human embryonic palate are highly responsive to epidermal growth factor. Science 213: 563-565, 1981. PubMed: 7017936

Yoneda T, Pratt RM. Interaction between glucocorticoids and epidermal growth factor in vitro in the growth of palatal mesenchymal cells from the human embryo. Differentiation 19: 194-198, 1981. PubMed: 6458523

Yoneda T, Pratt RM. Glucocorticoid receptors in palatal mesenchymal cells from the human embryo: relevance to human cleft palate formation. J. Craniofacial Genet. Dev. Biol. 1: 411-423, 1981.

Pratt RM, et al. Prescreening for environmental teratogens using cultured mesenchymal cells from the human embryonic palate. Teratog. Carcinog. Mutagen. 2: 313-318, 1982. PubMed: 6130630

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