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HEP G2/2.2.1

規格:

價格:

編號:B164594

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

HEP G2/2.2.1

商品貨號

B164594

Organism

Homo sapiens, human

Tissue

liver

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

hepatocellular carcinoma

Age

15 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Cell line was derived from the hepatocellular carcinoma cell line, HepG2 (ATCC HB-8065). The parental cells were stably transfected at passage 48 with a human cholesterol 7 alpha-hydroxylase (CYP7) minigene/Luciferase construct.

Clinical Data

15 years

Caucasian

male

Comments

The vector map shown in U.S. Patent 5,821,057 notes the luciferase reporter is under the control of a human cholesterol 7-alpha-hydroxylase (CYP7) regulatory minigene. The cells must be treated with a test compound that up-regulates expression of the minigene and luciferase. Additional information regarding the transfection and selection of these cells is provided in the depositor's publications, including US Patent 5,821,057.

(Chiang JY, Stroup D. Assay for agents that affect cholesterol 7alpha-hydroxylase expression and a characterization of its regulatory elements. US Patent 5,821,057 dated Oct 13 1998)

Complete Growth Medium

A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate and 1200 mg/L sodium bicarbonate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Note: These cells are slow to attach after subculture. Allow 4 to 5 days for reattachment.

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 9,13

D16S539: 12,13

D5S818: 11,12

D7S820: 10

THO1: 9

TPOX: 8,9

vWA: 17

Name of Depositor

Northeastern Ohio University College of Medicine

Deposited As

human

U.S. Patent Number

5,821,057

Passage History

References

Chiang JY, Stroup D. Assay for agents that affect cholesterol 7alpha-hydroxylase expression and a characterization of its regulatory elements. US Patent 5,821,057 dated Oct 13 1998

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B234686：Fistulina hepatica (Schaeffer : Fries) Fries

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