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HD168
HD168
規格:
價格:
編號:B164583
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HD168
商品貨號
B164583
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
The antibody is useful in studies of mouse class I antigens and T cell receptors.
The antibody reacts with the TL (thymus - leukemia antigen, product of the tla locus) mouse class I antigen.
Derivation
A rat was immunized with the TL+ mouse leukemia ASL1 (a spontaneous leukemia that developed in a strain A mouse). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with the TL (thymus - leukemia antigen, product of the tla locus) mouse class I antigen. The antibody is useful in studies of mouse class I antigens and T cell receptors.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse TL antigen
Cellular Products
immunoglobulin; monoclonal antibody; against mouse TL antigen
Tumorigenic
Yes
Effects
Yes, in pristane primed nu/nu mice
Comments
A rat was immunized with the TL+ mouse leukemia ASL1 (a spontaneous leukemia that developed in a strain A mouse). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with the TL (thymus - leukemia antigen, product of the tla locus) mouse class I antigen. The antibody is useful in studies of mouse class I antigens and T cell receptors.
Complete Growth Medium
Culture Medium: RPMI 1640 medium with 0.1 mM hypoxanthine, 0.016 mM thymidine,
90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2a
Name of Depositor
ES Stockert
Deposited As
rat (B cell); mouse (myeloma)
References

Obata Y, et al. Structural analysis of TL genes of the mouse. Proc. Natl. Acad. Sci. USA 82: 5475-5479, 1985. PubMed: 2410925

Obata Y, et al. Influence of 5' flanking sequences on TL and H-2 expression in transfected L cells. Proc. Natl. Acad. Sci. USA 85: 3541-3545, 1988. PubMed: 3368465

Hamasima N, et al. Expression of TL, H-2, and chimeric H-2/TL genes in transgenic mice: abnormal thymic differentiation and T-cell lymphomas in a TL transgenic strain. Proc. Natl. Acad. Sci. USA 86: 7995-7999, 1989. PubMed: 2813374

Morita A, et al. TL antigen as a transplantation antigen recognized by TL-restricted cytotoxic T cells. J. Exp. Med. 179: 777-784, 1994. PubMed: 8113675

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