Karyotype
Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22% HCC38 contains a homozygous deletion at 3p12. At least 45 distinct derivative chromosomes were detected in most metaphases, including two large metacentric markers which are approximately 1.5 times longer than a normal A group chromosome. Other derivative chromosomes: del(1)(q24); del(1)(p22); del(1)(p13); add(1)(p12); del(2)(p16); add(3)(q10); del(3)(q13); ?del(4)(q13) (two copies per cell); del(5)?(q23q33); der (7)(pter?q11::?hsr); del(7)(p?); del(9)(p12); add(9)(p10); ?der(11); add(12)(q24)(very long); add(14)(p11); add(17)(p12); der(18); der(X); plus approx. 17-24 markers of unknown origin per cell. This is a hyper-triploid human cell line with a modal chromosome number of 75. Homogeneously staining regions and dicentric chromosomes were observed. Every chromosome pair had a least one rearrangement. No normal X chromosomes were observed and Y chromosomes were absent by QM staining. The following structural rearrangements were observed in 30 metaphases: an acentric fragment in 2/30 metaphases, a minute in 3/30, a chromosome break in 3/30, a chromatid break in 5/30, a ring chromosome in 1/30, and double minutes in 11/30 (1-5 copies). Pulverized chromosomes were reported in 5% of metaphases. C-banding revealed that several of the large markers are dicentric. No normal X chromosomes were observed and Y chromosomes were absent by QM staining. Normal copies of chromosomes 2,6,11,13,16 and 20 were seen. Composite karyotype: 65-79<3n> der(X), -1,-1,-1, del(1)(q24), del(1)(p22),del(1)(p13),add(1)(p12),-2,del(2)(p16)x2,-3,-3,-3, ?add(3)(q10),del(3)(q13)x2,-4,-4,-4, ?del(4)(q13)x2,-5, -5,-5,del(5)?(q23q33)x2,-6,-6,-7,-7,-7,der (7)(pter?q11::?hsr), del(7)(p?)x2,-8,-8,-8,-9,-9,-9,del(9)(p12)x2,add(9)(p10)x2, -10,-10,-10,-11,?der(11),-12,-12,-12, add(12)(q24), -13,-13,-14,-14,-14,add(14)(p11)x2,-15,-15,-15,-17,-17,-17, add(17)(p12)x2,-18,-18,-18,der(18),-19,-19,-19,-21,-21, -22,-22,+17 to 24 mar[cp11].
