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HCC1419
HCC1419
規格:
價格:
編號:B164557
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HCC1419
商品貨號
B164557
Organism
Homo sapiens, human
Tissue
mammary gland; breast
培養基
Cell Type
Epithelial
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent, The line grows as large epithelial attached cells in island-like formation.
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
TNM stage IIIA, grade 3,primary ductal carcinoma
Age
42 years
Gender
female
Ethnicity
Hispanic
Storage Conditions
liquid nitrogen vapor temperature
Karyotype
aneuploid
Derivation
This cell line was initiated on 12/28/94 from a patient who received prior chemotherapy with no previous family history of breast cancer. The line took 9 months to establish.
Clinical Data
female
Hispanic
42 years
Receptor Expression
estrogen receptor, negative
progesterone receptor, negative
Oncogene
her2/neu +, p53 -
Genes Expressed
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Cellular Products
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Comments

The tumor was classified as TNM stage IIIA, grade 3, invasive ductal carcinoma with 5 out of 5 lymph node metastasis.?

The cells are poorly differentiated.?

The cells overexpress Her2-neu but are negative for expression of p53. HCC1419 is positive for the epithelial cell specific marker epithelial glycoprotein 2 (EGP2) and for cytokeratin 19.?

The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).


Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C
Subculture Ratio: 1:2 to 1:3
Medium Renewal: 2 to 3 times a week.
Note: The line grows as large epithelial attached cells in island-like formation.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 9,12
D16S539: 11,12
D5S818: 10,12
D7S820: 11
THO1: 6,9.3
TPOX: 8
vWA: 16,17
Name of Depositor
AF Gazdar, AK Virmani
Year of Origin
December 28, 1994
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

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