Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Complete Growth Medium
The base medium for this cell line is Eagle's Minimum Essential Medium (ATCC® No. 30-2003).
To make the complete growth medium, add the following components to the base medium:
Calf Serum (ATCC® No. 30-2030) to a final concentration of 10%.
This medium is formulated for use in 5% CO2.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
