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H1HeLa
H1HeLa
規格:
價格:
編號:B164526
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
H1HeLa
商品貨號
B164526
Organism
Homo sapiens, human
Tissue
cervix
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2  [Cells contain human papilloma virus (HPV-18)]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
adenocarcinoma
Age
31 years
Gender
female
Ethnicity
Black
Applications
The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al.
This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.
Derivation
The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).
Clinical Data
31 years
Black
female
HeLa Markers
Y
Genes Expressed
keratin
Cellular Products
keratin
Comments
The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).
This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.
The line is usually carried as monolayer cultures in Leibovitz's L-15 medium, but can be readily adapted to other media and to suspension culture (in media without Ca++).
The H-HeLa line was cured of a mycoplasma contamination by W.M. Lee in Rueckert's laboratory and the cured line was given the designation H1-HeLa.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Remove the medium and add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the culture to sit for 5 minutes at room temperature. Add fresh culture medium, aspirate and dispense into new flasks.
Culture Conditions

Atmosphere: air, 100%

Temperature: 37°C

STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor
RR Rueckert
Deposited As
Homo sapiens
References

Lee WM Ph.D. thesis, Univ. Wisconsin, 1991

Medappa KC, et al. On the structure of rhinovirus 1A. Virology 44: 259-270, 1971. PubMed: 4327717

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