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GSML
GSML
規格:
價格:
編號:B164522
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
GSML
商品貨號
B164522
Organism
Saimiri sciureus sciureus, monkey, Guyanese squirrel
Tissue
peripheral blood, mononuclear cells
培養基
Cell Type
B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transforme
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 Cells contain Epstein Barr viral DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender
male
Applications
GSML is a B lymphocyte cell line established in 2000 by transformation of mononuclear cells from male squirrel monkeys with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51.
Chromosome analysis of GSML cells confirm that they are from Guyanese squirrel monkey.
Storage Conditions
liquid nitrogen vapor phase
Derivation
GSML is a B lymphocyte cell line established in 2000 by transformation of mononuclear cells from male squirrel monkeys with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Clinical Data
GSML is a B lymphocyte cell line established in 2000 by transformation of mononuclear cells from male squirrel monkeys with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
male
Comments
GSML is a B lymphocyte cell line established in 2000 by transformation of mononuclear cells from male squirrel monkeys with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51.
FKBP51 is the common cause of glucocorticoid resistance.
Chromosome analysis of GSML cells confirm that they are from Guyanese squirrel monkey.
The Guyanese squirrel monkey and the Bolivian squirrel monkey differ in response to pathologic and pharmacologic stimuli.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml. Maintain cell density between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Population Doubling Time
24 hrs
Name of Depositor
JG Scammell
Deposited As
Saimiri sciureus sciureus
Year of Origin
2000
References

Scammell JG, et al. The origin of four squirrel monkey cell lines established by karyotype analysis. Cytogenet. Cell Genet. 93: 263-264, 2001. PubMed: 11528123

Scammell JG, et al. Overexpression of the FK506-binding immunophilin FKBP51 is the common cause of glucocorticoid resistance in three New World primates. Gen. Comp. Endocrinol. 124: 152-165, 2001. PubMed: 11703081

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