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當(dāng)前位置: 首頁(yè) > ATCC代理 > GR-96 B164516
GR-96
GR-96
規(guī)格:
價(jià)格:
編號(hào):B164516
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
GR-96
商品貨號(hào)
B164516
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with an extracellular epitope on the mouse receptor for gamma interferon.
Derivation
Animals were immunized with mouse gamma interferon receptor protein purified from solubilized WEHI-3 cells (see ATCC TIB-68).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse receptor for gamma interferon
Cellular Products
immunoglobulin; monoclonal antibody; against mouse receptor for gamma interferon
Comments
Animals were immunized with mouse gamma interferon receptor protein purified from solubilized WEHI-3 cells (see ATCC TIB-68).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with an extracellular epitope on the mouse receptor for gamma interferon.
It does not have blocking activity for interferon - receptor binding.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, supplemented with 0.1mM nonessential amino acids and 15 mM HEPES, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgA; kappa light chain
Name of Depositor
SW Russell
Deposited As
rat (B cell); mouse (myeloma)
References

Basu M, et al. A monoclonal antibody against the ligand binding site of the receptor for mouse interferon-gamma. J. Interferon Res. 9: 551-562, 1989. PubMed: 2477471

Basu M, et al. Purification and partial characterization of a receptor protein for mouse interferon gamma. Proc. Natl. Acad. Sci. USA 85: 6282-6286, 1988. PubMed: 2970637

LeClaire RD, et al. Characterization and use of monoclonal and polyclonal antibodies against the mouse interferon-gamma receptor. J. Leukocyte Biol. 51: 507-516, 1992. PubMed: 1376354

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