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GL1
GL1
規格:
價格:
編號:B164507
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
GL1
商品貨號
B164507
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the B7-2 (GL1) antigen present on activated mouse B cells.
B7-2 shows minimal expression on unstimulated B cells, but is expressed at high density on activated B cells.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse B7-2 antigen
Cellular Products
immunoglobulin; monoclonal antibody; against mouse B7-2 antigen
Comments
Animals were immunized with LPS activated DBA/2J mouse B cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the B7-2 (GL1) antigen present on activated mouse B cells.
The B7-2 antigen is a ligand for CTLA-4 (T cell activation molecule).
B7-2 shows minimal expression on unstimulated B cells, but is expressed at high density on activated B cells.
It is distinct from B7-1 (previously referred to as B7).
Complete Growth Medium
RPMI 1640 medium with 1% nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2a
Name of Depositor
G Lazlo, KS Hathcock
Deposited As
rat (B cell); mouse (myeloma)
References

Hathcock KS, et al. Identification of an alternative CTLA-4 ligand costimulatory for T cell activation. Science 262: 905-907, 1993. PubMed: 7694361

Freeman GJ, et al. Uncovering of functional alternative CTLA-4 counter-receptor in B7- deficient mice. Science 262: 907-909, 1993. PubMed: 7694362

Hancock WW, et al. Costimulatory function and expression of CD40 ligand, CD80, and CD86 in vascularized murine cardiac allograft rejection. Proc. Natl. Acad. Sci. USA 93: 13967-13972, 1996. PubMed: 8943044

Freeman GJ, et al. Cloning of B7-2: a CTLA-4 counter-receptor that costimulates human T cell proliferation. Science 262: 909-911, 1993. PubMed: 7694363

  • B188350:GL1
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