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The epithelial-like GH3 clone generates growth hormone at a greater rate than the GH1 cells and also produces prolactin.
Studies on the control of the production of these protein hormones by the GH3 cells have shown that hydrocortisone stimulates the production of growth hormone and inhibits prolactin production.
The cells have been adapted to growth in suspension culture using Eagle's minimum essential medium (spinner) supplemented with 15% horse serum and 2.5% FBS. Under these conditions the cells continue to produce both growth hormone and prolactin
Although the GH3 was not a direct derivative of the GH1 clone (ATCC CCL-82), it was initiated after only two additional animal passages from the primary culture from which the GH1 clone was established.
The cell line was submitted to the American Type Culture Collection in October, 1972 in the 10th subculture generation.
Bancroft FC, et al. Control of growth hormone production by a clonal strain of rat pituitary cells. Stimulation by hydrocortisone. J. Cell Biol. 43: 432-441, 1969. PubMed: 5389137
Tashjian AH Jr., et al. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology 82: 342-352, 1968. PubMed: 4951281
Tashjian AH Jr., et al. Production of both prolactin and growth hormone by clonal strains of rat pituitary tumor cells. J. Cell Biol. 47: 61-70, 1970. PubMed: 5513559
Bancroft FC, Tashjian AH Jr.. Growth in suspension culture of rat pituitary cells which produce growth hormone and prolactin. Exp. Cell Res. 64: 125-128, 1971. PubMed: 5541958
Bancroft FC, Tashjian AH Jr.. Control of the production of two protein hormones by rat pituitary cells in culture. In Vitro 6: 180-189, 1970. PubMed: 5535574
Wood WM, et al. Thyroid hormone receptor beta2 promoter activity in pituitary cells is regulated by Pit-1. J. Biol. Chem. 271: 24213-24220, 1996. PubMed: 8798664
