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G-3-5
G-3-5
規格:
價格:
編號:B164481
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
G-3-5
商品貨號
B164481
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Autopsy
Applications
It is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Animals were immunized human brain homogenate of multiple sclerosis plaques obtained at autopsy.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against alpha actinin
Cellular Products
immunoglobulin; monoclonal antibody; against alpha actinin
Comments
Animals were immunized human brain homogenate of multiple sclerosis plaques obtained at autopsy.
Spleen cells were fused with NS-1 myeloma cells.
The antibody recognizes a novel intermediate filament associated protein (MW 100000) with similarity to alpha actinin.
This antibody intensely stains astroglia and related macroglia in cryostat sections of human and rat central nervous system tissue.
It is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cultures at a cell concentration between 0.5 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
mouse IgM
Name of Depositor
SK Malhotra
Deposited As
mouse (B cell); mouse (myeloma)
References

Jeffcoat S, et al. A novel intermediate filament-associated protein: further characterization of the G.3.5 antigen. Cytobios 82: 81-99, 1995. PubMed: 7587376

. . Ann. N.Y. Acad. Sci. 436: 492-494, 1984.

Price KA, et al. Localization and characterization of an intermediate filament- associated protein. Cytobios 76: 157-173, 1993. PubMed: 8181306

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