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G28-5
G28-5
規(guī)格:
價(jià)格:
編號(hào):B164477
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
G28-5
商品貨號(hào)
B164477
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody is specific for the Bp50 (CD40) B cell surface antigen.
The antibody reacts specifically with CD20 positive (Bp35, B1) blood and tonsillar B cells, and does not react with T cells or NK cells.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Bp50 (CD40, human B cell antigen)
Cellular Products
immunoglobulin; monoclonal antibody; against Bp50 (CD40, human B cell antigen)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with human tonsillar lymphocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody is specific for the Bp50 (CD40) B cell surface antigen.
The antibody reacts specifically with CD20 positive (Bp35, B1) blood and tonsillar B cells, and does not react with T cells or NK cells.
It does react with malignant B cells and B cell lines.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or by replacement of medium. Alternatively, cultures can be established by centrifigugation with subsequent resuspension at 2 X 10(5) viable cells/ml. Maintain between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Oncogen
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Ledbetter JA, Clark EA. Ligands and methods for augmenting B-cell proliferation. US Patent 5,182,368 dated Jan 26 1993

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