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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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當前位置: 首頁 > ATCC代理 > FW3-218-1 [218] B164475
FW3-218-1 [218]
FW3-218-1 [218]
規格:
價格:
編號:B164475
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
FW3-218-1 [218]
商品貨號
B164475
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with and precipitates sheep alpha 4 integrin.
It cross-reacts with bovine alpha 4 integrin, but not with the pig, rabbit or human counterparts.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against sheep (ovine) alpha 4 integrin (VLA-4, CD49d)
Cellular Products
immunoglobulin; monoclonal antibody; against sheep (ovine) alpha 4 integrin (VLA-4, CD49d)
Comments
Animals were immunized with sheep afferent lymph lymphocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with and precipitates sheep alpha 4 integrin.
It cross-reacts with bovine alpha 4 integrin, but not with the pig, rabbit or human counterparts.
Complete Growth Medium
CULTURE MEDIUM: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2b
Name of Depositor
CR Mackay
Deposited As
mouse (B cell); mouse (myeloma)
References

Mackay CR, et al. Tissue-specific migration pathways by phenotypically distinct subpopulations of memory T cells. Eur. J. Immunol. 22: 887-895, 1992. PubMed: 1372559

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