Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain
Strain:? ? (B cell); BALB/c (myeloma)
Applications
The antibody is protective against tularemia in animal models using the LVS strain.
It cross-reacts with wild-type F. tularensis.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Francisella tularensis lipopolysaccharide
Cellular Products
immunoglobulin; monoclonal antibody; against Francisella tularensis lipopolysaccharide
Comments
Animals were immunized with live F. tularensis (LVS strain).
Spleen cells were fused with mouse myeloma cells.
The antibody is protective against tularemia in animal models using the LVS strain.
It cross-reacts with wild-type F. tularensis.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell density to exceed 1 X 10(6) cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
