亚洲激情在线,成人免费精品视频,99国产免费

FHC

規格:

價格:

編號:B164454

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

FHC

商品貨號

B164454

Organism

Homo sapiens, human

Tissue

colon

培養基

Cell Type

Epithelial

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

13 weeks gestation

Storage Conditions

liquid nitrogen vapor phase

Images

Comments

When using a non-humidified incubator, gassing of cultures with 5% CO2 and plug-seal caps should be used to prevent evaporation of liquid in media and unintentional concentrating of media additives.

Although FHC cells exhibit epithelial morphology, cytoplasmic keratins were not detected.

Complete Growth Medium

The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium, add the following components to the base medium:

extra 10 mM HEPES (for a final conc. of 25 mM)
10 ng/ml cholera toxin
0.005 mg/ml insulin
0.005 mg/ml transferrin
100 ng/ml hydrocortisone
20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)
Fetal Bovine Serum, 10% final conc (ATCC 30-2020)

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:

extra 10 mM HEPES (for a final conc. of 25 mM)
10 ng/ml cholera toxin
0.005 mg/ml insulin
0.005 mg/ml transferrin
100 ng/ml hydrocortisone
fetal bovine serum 10%(final conc.

Subculturing

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:

extra 10 mM HEPES (for a final conc. of 25 mM)
10 ng/ml cholera toxin
0.005 mg/ml insulin
0.005 mg/ml transferrin
100 ng/ml hydrocortisone
fetal bovine serum 10%(final conc.

Cryopreservation

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 4 to 6 X 10³ viable cell/cm² is recommended.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended every 10 to 15 days

Medium Renewal: Every 3 to 4 days.

Culture Conditions

Freeze Medium: Complete growth medium supplemented with an extra 50% FBS and 10% (v/v) DMSO

Storage Temperature: Liquid nitrogen vapor phase

STR Profile

Temperature: 37°C

Atmosphere: Air, 95%; carbon dioxide (CO₂), 5%

Name of Depositor

Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 12,13

D16S539: 9,11

D5S818: 12,13

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,11

vWA: 16

Deposited As

DP Chopra

References

Homo sapiens

B164456：FHCR-1-2813/FDC-6

B164455：FHCR-1-2624/FH6/FHOT-1-3019 [FH6]

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479