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FeT-1C
FeT-1C
規(guī)格:
價(jià)格:
編號:B164452
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
FeT-1C
商品貨號
B164452
Organism
Felis catus, cat
Tissue
peripheral blood mononuclear cells (PBMC); T lymphocyte
培養(yǎng)基
Cell Type
T lymphocyte
Product Format
frozen
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
The cell line was derived from a specific pathogen free (SPF) cat [U.S. Pat. 5,846,825].
Virus Susceptibility
Feline immunodeficiency virus
Comments

The FeT-1C cell line (ATCC CRL-11968) is IL-2 dependent, whereas the FeT-J cell line (ATCC CRL-11967) is IL-2 independent [U.S. Pat. 5,846,825] This feline T-cell line is susceptible to infection by feline immunodeficiency virus (FIV) [U.S. Pat. 5,846,825].


Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100 Units/ml human recombinant IL-2 and 10% heat-inactivated fetal bovine serum
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor
University of Florida, Gainesville
Deposited As
cat
U.S. Patent Number
References

Yamamoto JK. Feline derived T-cell lines for producing FIV. US Patent 5,846,825 dated Dec 8 1998

Hohdatsu T, et al. Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence. J. Gen. Virol. 77: 93-100, 1996. PubMed: 8558132

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