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FDC-P1

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價格:

編號:B164449

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

FDC-P1

商品貨號

B164449

Organism

Mus musculus, mouse

Tissue

bone marrow

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Normal

Strain

DBA/2

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor temperature

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Karyotype

diploid

Derivation

The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells (see ATCC TIB-68).

Antigen Expression

Thy-1.2 (24%)

Comments

They are dependent upon WEHI-3 conditioned medium (WEHI-3CM) for continued growth.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:

mouse Interleukin-3 culture supplement (BD Biosciences, Catalog No. 354040) 25% (final conc.)
fetal bovine serum 10% (final conc.)

To make a 500 mL volume:

325 mL DMEM (ATCC 30-2002)
125 mL Mouse Interleukin-3 (BD 354040)
50 mL FBS (ATCC 30-2020)

This medium is formulated for use with a 5% CO₂in air atmosphere. Standard DMEM formulations contain 3.7 g/L sodium bicarbonate. A 10% CO₂in air atmosphere is recommended.

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10⁵ cells/mL and maintain between 1 X 10⁵ and 1 X 10⁶ cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium, 90%; DMSO, 10%

Storage temperature: liquid nitrogen vapor temperature

Culture Conditions

Temperature: 37°C

Name of Depositor

University of Virginia Patent Foundation

Deposited As

Mus musculus

U.S. Patent Number

7,125,659

References

Dexter TM, et al. Growth of factor-dependent hemopoietic precursor cell lines. J. Exp. Med. 152: 1036-1047, 1980. PubMed: 6968334

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