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EL4.BU
EL4.BU
規格:
價格:
編號:B164405
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
EL4.BU
商品貨號
B164405
Organism
Mus musculus, mouse
Cell Type
T lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
C57BL/6N
Applications
This line was derived from the EL4 cell line (see ATCC TIB-39).
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine (BUdR).
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line was derived from the EL4 cell line (see ATCC TIB-39).
Antigen Expression
H-2b; Thy-1.2
Genes Expressed
H-2b; Thy-1.2
Comments
This line was derived from the EL4 cell line (see ATCC TIB-39).
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine (BUdR).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor
R Hyman
Deposited As
Mus musculus
References

Mohit B, Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science 171: 75-77, 1971. PubMed: 4099030

Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956

Kohler G, et al. Fusion of T and B cells. Somatic Cell Genet. 3: 303-312, 1977. PubMed: 305123

Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.

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