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EJG

規格:

價格:

編號:B164401

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

EJG

商品貨號

B164401

Organism

Bos taurus, cow

Tissue

adrenal gland

培養基

Cell Type

endothelial

Product Format

frozen

Morphology

endothelial

Culture Properties

adherent

Biosafety Level

2 [Cells contain Bovine Viral Diarrhea Virus (BVDV)]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

The line was derived from collagenase dissociated bovine adrenal tissue.

Genes Expressed

Factor VIII:C

Cellular Products

Factor VIII:C

Comments

The cellular morphology is that of capillary endothelium.

The cells form linearly oriented confluent cultures.

Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for a 75 cm2 flask? proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of TrypsinEDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension (75 sq. cm. with 1 X 10⁴ cells) to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week

Culture Conditions

Temperature: 37°C

Population Doubling Time

110 hrs

Name of Depositor

The United States of America

U.S. Patent Number

4,670,394

References

Pollard HB, et al. Isolation and culture of adrenal medullary endothelial cells producing blood clotting factor VIII:C. US Patent 4,670,394 dated Jun 2 1987

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

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