Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with both IgG2a and IgG2b.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified mouse IgG2b (lambda).
Lymph node cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse IgG2
Cellular Products
immunoglobulin; monoclonal antibody; against mouse IgG2
Comments
Animals were immunized with purified mouse IgG2b (lambda).
Lymph node cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with both IgG2a and IgG2b.
Tested and found negative for ectromelia virus (mousepox).
A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (ATCC 56-X) is necessary for successful initiation of a culture from a thawed ampule.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(5) viable cells/ml into new culture vessels pre-plated with ATCC 56-X feeder layer (irradiated STO cells). Maintain between 1 X 10 (5) and 1 X 10 (6) cells/ml.
