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E.G7-OVA [derivative of EL4]

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價格:

編號:B164385

品牌:Mingzhoubio

標準菌株

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DNA

RNA

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凍干粉

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甘油

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產品名稱

E.G7-OVA [derivative of EL4]

商品貨號

B164385

Organism

Mus musculus, mouse

Tissue

lymphocyte

培養基

Cell Type

T lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

lymphoma

Strain

C57BL/6N

Applications

This cell line is a model system for studying major histocompatibility complex (MHC) class I restricted responses of cytotoxic T lymphocytes in mice.

Storage Conditions

liquid nitrogen vapor phase

Derivation

E.G7-OVA was derived in 1988 from the C57BL/6 (H-2 b) mouse lymphoma cell line EL4 (ATCC TIB-39).

The EL4 cells were transfected by electroporation with the plasmid pAc-neo-OVA which carries a complete copy of chicken ovalbumin (OVA) mRNA and the neomycin (G418) resistance gene.

Antigen Expression

H-2 b

Genes Expressed

chicken ovalbumin

Comments

E.G7-OVA cells contain a single copy of the inserted plasmid and synthesize and secrete OVA constitutively.

C57BL/6 mice immunized with E.G7-OVA cells give rise to H-2 Kb restricted cytotoxic lymphocytes specific for the OVA 258-276 peptide.

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol and 0.4 mg/ml G418, 90%; fetal bovine serum, 10%

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10⁵cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Culture medium, 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

Name of Depositor

MJ Bevan

Deposited As

Mus musculus

Year of Origin

1988

References

Moore MW, et al. Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell 54: 777-785, 1988. PubMed: 3261634

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