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DSN
DSN
規(guī)格:
價(jià)格:
編號(hào):B164375
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
DSN
商品貨號(hào)
B164375
Organism
Canis familiaris, dog
Tissue
bone; Derived from metastatic site: lung
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 Cells contain CMV, SNV, SV-40 viral DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
osteosarcoma
Age
11 years
Gender
female
Strain
poodle
Applications
The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus.
The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus.
The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus.
Clinical Data
female
Comments
The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus.
The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin-0.02% EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 4 to 6 days.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
Wisconsin Alumni Res. Fndn.
Deposited As
Canis familiaris
U.S. Patent Number
References

Temin HM, Dougherty JP. Recombination resistant retroviral helper cell and products produced thereby. US Patent 5,124,263 dated Jun 23 1992

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