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DREG200
DREG200
規格:
價格:
編號:B164367
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
DREG200
商品貨號
B164367
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
It cross-reacts with rabbit L-selectin.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human L-selectin (CD62L, LECAM, LAM-1, Leu8)
Cellular Products
immunoglobulin; monoclonal antibody; against human L-selectin (CD62L, LECAM, LAM-1, Leu8)
Comments
Animals were immunized with supernatant from phorbol myristic acid activated human peripheral blood leukocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody partially blocks lymphocyte adhesion to high enothelial venules of peripheral lymph nodes.
It cross-reacts with rabbit L-selectin.
Complete Growth Medium
Culture Medium: RPMI 1640 medium with 4.5 g/L glucose, 10 mM HEPES, 2 mM
L-glutamine, 1 mM sodium pyruvate and 0.1 mM non-essential amino acids
supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG1
Name of Depositor
EC Butcher
Deposited As
mouse (B cell); mouse (myeloma)
References

Kishimoto TK, et al. Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc. Natl. Acad. Sci. USA 87: 2244-2248, 1990. PubMed: 2179952

von Andrian UH, et al. Two-step model of leukocyte-endothelial cell interaction in inflammation: distinct roles for LECAM-1 and the leukocyte beta 2 integrins in vivo. Proc. Natl. Acad. Sci. USA 88: 7538-7542, 1991. PubMed: 1715568

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