Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age
newborn
Gender
female
Ethnicity
Caucasian
Karyotype
B/D translocation; 45,XX,t(Bp-;Dq+); translocation involves chromosomes 5 and 14
Clinical Data
female
Caucasian
newborn
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let culture stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
