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Daoy

規格:

價格:

編號:B164331

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Daoy

商品貨號

B164331

Organism

Homo sapiens, human

Tissue

brain/cerebellum

培養基

Product Format

frozen

Morphology

polygonal

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

desmoplastic cerebellar medulloblastoma

Age

4 years

Gender

male

Ethnicity

Caucasian

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

This is a hypertetraploid human cell line with a modal number between 93 and 99. The frequency of cells with higher ploidies is 2.0%. However, since the stemline chromosome number is high, the estimate for the polyploidy is tentative. Thirteen or more marker chromosomes were common to all cells. Of these, many had two to four copies per cell. Among the markers were: t(1q5q), t(13q;?), 15p+, 7q+, der(9)t(3;9)(p21;q34) and eight others. In most cells, the 15p+ has three copies and der(9) has four copies.Some cells have del(1)(p11). Normal N12, N14, N15 and N19 tend to have four or more copies per cell. There are two normal X chromosomes in most cells, but there is no detectable normal Y.

Images

Derivation

The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia.

The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy.

Clinical Data

Caucasian

male

The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy.

4 years

Tumorigenic

Yes

Effects

Yes, in nude mice

(The cells form serially transplantable intercranial and subcutaneous tumors.)

Comments

Although the original tumor had characteristics of both neuronal and glial differentiation, these were not retained by the cell line.

Treatment of the cells with dibutyryl cyclic amp (cAMP) does not induce expression of those characteristics as measured by staining for S100 (S-100) protein and glial fibrillary acidic proteins (GFAP).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 11

D13S317: 13,14

D16S539: 10

D5S818: 11,13

D7S820: 8,10

THO1: 9

TPOX: 8,10

vWA: 14,20

Isoenzymes

AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 1

Me-2, 1

PGM1, 2

PGM3, 1-2

Population Doubling Time

34 hrs

Name of Depositor

HS Friedman

Deposited As

Homo sapiens

Year of Origin

1985

References

He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373

Jacobsen PF, et al. Establishment of a human medulloblastoma cell line and its heterotransplantation into nude mice. J. Neuropathol. Exp. Neurol. 44: 472-485, 1985. PubMed: 2993532

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

The cells form serially transplantable intercranial and subcutaneous tumors.

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