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D8/17

規格:

價格:

編號:B164320

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

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產品名稱

D8/17

商品貨號

B164320

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: ?BALB/c (B cell); BALB/c (myeloma)

Applications

The antibody detects an antigen present on 95% of patients susceptible to rhematic fever.

Elevated levels of D8/17 antibodies may represent a marker of susceptibility to "pediatric autoimmune neuropsychiatric disorders associated with streptococcal infections" (PANDAS).

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with P3X63Ag8.U1 myeloma cells.

Clinical Data

The antibody detects an antigen present on 95% of patients susceptible to rhematic fever.

Genes Expressed

immunoglobulin; monoclonal antibody; against a human B cell alloantigen associated with rheumatic fever

Cellular Products

immunoglobulin; monoclonal antibody; against a human B cell alloantigen associated with rheumatic fever

Tumorigenic

Yes

Effects

Yes, forms ascites in BALB/c mice

Comments

Animals were immunized with human B lymphocytes.

Spleen cells were fused with P3X63Ag8.U1 myeloma cells.

The antibody detects an antigen present on 95% of patients susceptible to rhematic fever.

It did not react with other leukocyte antigens tested (HLA A, B, C, and DR1 through DR8).

Elevated levels of D8/17 antibodies may represent a marker of susceptibility to "pediatric autoimmune neuropsychiatric disorders associated with streptococcal infections" (PANDAS).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Subculturing

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Culture Conditions

Temperature: 37.0°C

Isotype

IgM; kappa light chain

Name of Depositor

Rockefeller Univ.

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,743,538

References

Zabriskie JB, Buskirk DR. Test for rheumatic fever and monoclonal antibodies useful therefor. US Patent 4,743,538 dated May 10 1988

Arnold PD, Richter MA. Is obsessive-compulsive disorder an autoimmune disease?. CMAJ 165: 1353-1358, 2001. PubMed: 11760984

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