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D341 Med
D341 Med
規格:
價格:
編號:B164319
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
D341 Med
商品貨號
B164319
Organism
Homo sapiens, human
Tissue
brain/cerebellum
培養基
Cell Type
Medulloblastoma
Product Format
frozen
Morphology
spheroid
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
medulloblastoma
Age
3.5 years
Gender
male
Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a hyperdiploid (modal number = 49; range = 44 to 50) human cell line with higher ploidies occurring with a frequency of 8.5%. Four marker chromosomes are present in all cells. They are: 11HSR, i(17q), der(22)t(1;22)(q12;p12) and 8q-. There are three copies each of N6 and N18, and single copies each of N11, N17 and N22. The single X chromosome was structurally normal, and the presence of the Y chromosome was confirmed by fluorescence microsopy.
Images
Derivation
The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma.
Clinical Data
The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma.
male
Genes Expressed
The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody.
Cellular Products
neurofilament proteins
Tumorigenic
Yes
Effects
Yes, in nude mice; forms serially transplantable intercranial and subcutaneous tumors
Yes, in soft agar
Comments
The cells produce tumors in nude mice.
The cell line is positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
The cells are positive for the neuroectodermal antigen recognized by the UJ13A monoclonal antibody.
The c-myc oncogene is amplified in D341 Med cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by the addition of fresh medium. Cells grow mostly as floating aggregates of irregular-shaped cell clusters with some attached cells. Clusters are alive while single cells have very low viability. Attached cells can be shaken loose.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,13
D16S539: 12,14
D5S818: 11,12
D7S820: 9,13
THO1: 6,9.3
TPOX: 8,11
vWA: 17,18
Population Doubling Time
37 hrs
Name of Depositor
HS Friedman
Deposited As
Homo sapiens
Year of Origin
1988
References

Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793

He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

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