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D-17
D-17
規格:
價格:
編號:B164314
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
D-17
商品貨號
B164314
Organism
Canis familiaris, dog
Tissue
bone; derived from metastatic site: lung
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
osteosarcoma
Age
11 years
Gender
female
Strain
poodle
Applications
This cell line may be used as a transfection host.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 63; range = 58 to 70.
There were 25 to 30 biarmed chromosomes not common to the diploid dog karyotype present in all cells examined. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines
Images
Derivation
Derived from an osteosarcoma metastatic to the lung in an 11-year-old female poodle.
Tumorigenic
Yes
Effects
Yes, in immunosuppressed mice
Yes, in nude mice
Virus Susceptibility
Canine adenovirus 1
Canid herpesvirus 1 , Canine herpesvirus
Canine parainfluenza virus
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor
WA Nelson-Rees
Deposited As
Canis familiaris
Year of Origin
1969
References

Temin HM, Watanabe S. Helper cell. US Patent 4,650,764 dated Mar 17 1987

Riggs JL, et al. Immunofluorescent studies of RD-114 virus replication in cell culture. J. Gen. Virol. 25: 21-29, 1974. PubMed: 4372315

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