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D1 ORL UVA [D1]

規格:

價格:

編號:B164310

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

D1 ORL UVA [D1]

商品貨號

B164310

Organism

Mus musculus, mouse

Tissue

bone marrow

培養基

Product Format

frozen

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

BALB/c

Applications

This cell line is a suitable transfection host.

The cells may be systemically administered to treat osteoporosis, osteolysis, improve bone implant adherence, augment bone growth or bone repair, augment cartilage repair, and augment fat production.

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

The D1 cell line was cloned from a multipotent mouse bone marrow stromal precursor.

Clinical Data

The cells are "homing" cells, that is, cells injected systemically into the patient home to the bone marrow, unless specifically placed in another location.

Comments

The cells can differentiate into osteocytes, chondrocytes and adipocytes in the presense of the appropriate stimulus and environment.

The cells are "homing" cells, that is, cells injected systemically into the patient home to the bone marrow, unless specifically placed in another location.

The cells may be transformed with recombinant DNA for the expression of both reporter genes, and biological factors, such as growth factors.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Name of Depositor

University of Virginia

Deposited As

mouse

U.S. Patent Number

6,082,364

References

Balian G, et al. Pluripotential bone marrow cell line and methods of using the same. US Patent 6,082,364 dated Jul 4 2000

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