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CPAE

規格:

價格:

編號:B164292

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

CPAE

商品貨號

B164292

Organism

Bos taurus, cow

Tissue

pulmonary artery/endothelium

培養基

Cell Type

endothelial

Product Format

frozen

Morphology

endothelial

Culture Properties

adherent

Biosafety Level

2 [Cells contain Bovine Viral Diarrhea Virus (BVDV)]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Gender

female

Storage Conditions

liquid nitrogen vapor phase

Karyotype

normal female; diploid; stable

Images

Derivation

CPAE is an endothelial cell line derived from the main stem pulmonary artery of a young cow (Bos taurus). This line was initiated in January, 1979 by P. Del Vecchio from artery lumen scrapings dispersed by enzyme treatment.

Clinical Data

female

Genes Expressed

positive for angiotensin converting enzyme

Cellular Products

positive for angiotensin converting enzyme

Comments

Subsequent to the publication of the sixth edition of the ATCC catalog, tests for bovine diarrhea virus (BVD) have indicated that CPAE cells test positive for BVD viral antigen.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

Atmosphere: 95% air; 5% CO₂

Name of Depositor

PJ Del Vecchio

Deposited As

Bos taurus

Year of Origin

1979

References

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Girard JP, Springer TA. Modulation of endothelial cell adhesion by hevin, an acidic protein associated with high endothelial venules. J. Biol. Chem. 271: 4511-4517, 1996. PubMed: 8626806

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