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Con A - C1 - VICK
Con A - C1 - VICK
規格:
價格:
編號:B164289
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Con A - C1 - VICK
商品貨號
B164289
Organism
Gallus gallus, chicken
Tissue
spleen
培養基
Cell Type
T lymphocyte; transformed with reticuloendotheliosis virus (ATCC VR-770
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, multicell aggregates, clusters in suspension
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
42 week old
Gender
female
Applications
Two immortal cell lines were established, CON A-C1-VICK (ATCC CRL-12135) and ConA-B1-VICK (ATCC CRL-12357).
Both cell lines produce granulocyte colony stimulating factor (G-CSF).
When cultured in vitro, the cell lines produce and secrete immune lymphokines that may be administered to fowl to increase their resistance to infections.
The cell lines produce lymphokines which, following administration into either 18 day old chick embryos or day of hatch chicks, prevents extraintestinal Salmonella infection.
Neither the lymphokine or cell line induces viral pathogenesis in chickens.
For lymphokine production, remove cells from maintenance medium, wash in serum-free RPMI, culture at 5 x 10 exp6 cells/ml in serum-free RPMI containing 0.0075 mg/ml Concanavalin A (ConA) for 72 hours.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleens were harvested and T cells were isolated.
Clinical Data
female
Genes Expressed
granulocyte colony stimulating factor (G-CSF)
Cellular Products
granulocyte colony stimulating factor (G-CSF)
Comments
Animals were immunized with Salmonella enteritidis.
Spleens were harvested and T cells were isolated.
T cells were incubated with Concanavalin A and subsequently transformed with Avian reticuloendotheliosis virus (REV-T with CSV)(ATCC VR-770).
Two immortal cell lines were established, CON A-C1-VICK (ATCC CRL-12135) and ConA-B1-VICK (ATCC CRL-12357).
Both cell lines produce granulocyte colony stimulating factor (G-CSF).
When cultured in vitro, the cell lines produce and secrete immune lymphokines that may be administered to fowl to increase their resistance to infections.
The cell lines produce lymphokines which, following administration into either 18 day old chick embryos or day of hatch chicks, prevents extraintestinal Salmonella infection.
Neither the lymphokine or cell line induces viral pathogenesis in chickens.
For lymphokine production, remove cells from maintenance medium, wash in serum-free RPMI, culture at 5 x 10 exp6 cells/ml in serum-free RPMI containing 0.0075 mg/ml Concanavalin A (ConA) for 72 hours.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: 2 to 3 times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor
USDA/ARS
Deposited As
chicken
U.S. Patent Number
References

Kogut MH, et al. Method to produce granulocyte colony stimulating factor from immortalized avian T lymphocytes and method to produce immortalized cells. US Patent 5,691,200 dated Nov 25 1997

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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