最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > COLO 320HSR [COLO 320 HSR] B164285
COLO 320HSR [COLO 320 HSR]
COLO 320HSR [COLO 320 HSR]
規格:
價格:
編號:B164285
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
COLO 320HSR [COLO 320 HSR]
商品貨號
B164285
Organism
Homo sapiens, human
Tissue
colon
培養基
Product Format
frozen
Morphology
cells are rounded and refractile
Culture Properties
loosely adherent, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Dukes' type C, colorectal adenocarcinoma
Age
55 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
marker chromosomes with homogeneously staining regions (HSR) were observed and the double minute (DM) chromosomes appeared in much lower frequency than the parental line COLO 320DM
Clinical Data
55 years
Caucasian
female
Genes Expressed
serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone
Cellular Products
serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone
Tumorigenic
Yes
Effects
Yes, in nude mice
Comments
The cells are weakly positive for keratins and vimentin.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Shake flask. Remove culture medium to a centrifuge tube.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 x g for 5 to10 minutes.
  6. Discard supernatant and resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new culture vessels.
  7. Place culture vessels in incubators at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: FreezeMedium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 8,9
TPOX: 8,9
vWA: 15,18
Isoenzymes
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 2
Name of Depositor
GE Moore
Deposited As
Homo sapiens
References

Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479