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COLO 201

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價格:

編號:B164282

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

COLO 201

商品貨號

B164282

Organism

Homo sapiens, human

Tissue

colon; derived from metastatic site: ascites

培養基

Product Format

frozen

Morphology

bipolar, slightly refractile, fibroblast-like cell

Culture Properties

suspension, with some loosely adherent cells

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Dukes' type D, colorectal adenocarcinoma

Age

70 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.

Derivation

This cell line was derived from tissue from the same patient as COLO 205 (ATCC CCL-222).

Clinical Data

70 years

Caucasian

male

Oncogene

myc +; ras + ; myb + ; fos +; sis +; p53 +; abl -; ros -; src -

Tumorigenic

Yes

Effects

Yes, in nude mice

(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)

Comments

The line is negative for CSAp (CSAp-) and CEA.

This cell line is tumorigenic in mice. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10⁷ cells

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a 0.25% trypsin, 0.53 mM EDTA solution. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended

Medium Renewal: 1 to 2 times per week

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 11,12

D13S317: 10,12

D16S539: 12,13

D5S818: 10,13

D7S820: 9,10

THO1: 8,9

TPOX: 11

vWA: 15

Isoenzymes

ES-D, 1-2

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1-2

PGM3, 1-2

Name of Depositor

GE Moore

Deposited As

Homo sapiens

References

Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

B244838：Trametes versicolor (Linnaeus : Fries) Lloyd

B244831：Aspergillus versicolor (Vuillemin) Tiraboschi

B244475：Penicillium olivicolor Pitt, anamorph

B244301：Laccaria bicolor (Maire) Orton

B242483：Teunomyces barrocoloradensis (Suh et al.) Kurtzman et Blackwell

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