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CMH1a

規格:

價格:

編號:B164277

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

CMH1a

商品貨號

B164277

Organism

Mus musculus, mouse

Tissue

mammary gland

培養基

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Papilloma, hepatitis

Strain

RIII

Applications

This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.

HBsAg production is under the control of the metallothionine promoter.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.

HeLa Markers

Genes Expressed

surface antigen [sAg]

Comments

This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.

HBsAg production is under the control of the metallothionine promoter.

The construct contains the entire BPV genome and the hepatitis B sAg structural gene inserted within the MT gene between the promoter and the rest of the gene.

A BamHI/SalI fragment of pRF300 (containing hepatitis B sAg and MT-1 promoter) was ligated to a BamHI/SalI fragment of pB2-2 (containing the entire BPV genome and pBR sequences) to create pRB-1.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:15 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature (or 37C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense, into new flasks.

Cryopreservation

fetal bovine serum, 90%; glycerol, 10%

Culture Conditions

Temperature: 37.0°C

Name of Depositor

Integrated Genetics, Inc.

Deposited As

Mus musculus

U.S. Patent Number

4,696,898

References

Fitts RA, et al. Vector encoding hepatitis B surface antigen. US Patent 4,496,898 dated Sep 29 1987

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