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Clone M-3 [Cloudman S91 melanoma]
Clone M-3 [Cloudman S91 melanoma]
規格:
價格:
編號:B164274
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Clone M-3 [Cloudman S91 melanoma]
商品貨號
B164274
Organism
Mus musculus, mouse
Tissue
skin
培養基
Cell Type
melanocyte
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
melanoma
Gender
male
Strain
DBA
Storage Conditions
liquid nitrogen vapor phase
Karyotype
Stemline number is hypertetraploid. Karyotype is stable within stemline number. Marker chromosomes: A medium size chromosome with a submedian centromere and a smaller chromosome with a median centromere., The remaining 81 chromosomes have terminal centromeres, the first one is larger than normal. A minute chromosome was noted in 20% of the cells.
Derivation
Clone M-3, a melanin-producing cell line was adapted to cell culture by Y. Yasumura, A.H. Tashjian and G. Sato from a Cloudman S91 melanoma in a (C X DBA) F1 male mouse obtained from the Jackson Memorial Laboratory, Bar Harbor, Maine.
Clinical Data
male
Genes Expressed
melanin
Cellular Products
melanin
Tumorigenic
Yes
Effects
Yes, in C57BL mice
Virus Susceptibility
Herpes simplex virus
Vaccinia virus
Pseudorabies virus
Vesicular stomatitis, Glasgow (Indiana)
Vesicular stomatitis, Orsay (Indiana)
Virus Resistance
poliovirus 1
Comments

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium
The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 2.5%
  • horse serum to a final concentration of 15%
    • Subculturing

    Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

    1.  Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin 0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37oC to facilitate dispersal. 
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
    5. Add appropriate aliquots of the cell suspension into new culture vessels.
    6. Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

    Medium Renewal: Every other day

    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37°C
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Name of Depositor
    G Sato
    Deposited As
    Mus musculus
    References

    Yasamura Y, et al. Establishment of four functional, clonal strains of animal cells in culture. Science 154: 1186-1189, 1966. PubMed: 4288399

    Schmidt W, et al. Cell-free tumor antigen peptide-based cancer vaccines. Proc. Natl. Acad. Sci. USA 94: 3262-3267, 1997. PubMed: 9096381

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