日韩久久综合,一二三四区,久色在线

CKMM 14.52

規格:

價格:

編號:B164262

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

CKMM 14.52

商品貨號

B164262

Organism

Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)

Tissue

spleen

培養基

Cell Type

hybridoma: B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The antibody reacts preferentially with CK-MM, but cross-reacts with CK-MB.

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against human CK-MM (creatine kinase - MM)

Cellular Products

immunoglobulin; monoclonal antibody; against human CK-MM (creatine kinase - MM)

Comments

Animals were immunized with human creatine kinase - MM.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts preferentially with CK-MM, but cross-reacts with CK-MB.

Binding of the antibody inhibits the enzyme activity of both CK-MM and CK-MB.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.

Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Isotype

IgG1 kappa

Name of Depositor

Eastman-Kodak Co.

Deposited As

rat (B cell); mouse (myeloma)

U.S. Patent Number

4,950,592

References

Daiss JL. Blend of monoclonal antibodies. US Patent 4,950,592 dated Aug 21 1990

B164261：CKMM 14.15

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