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Ch13
Ch13
規(guī)格:
價格:
編號:B164242
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Ch13
商品貨號
B164242
Organism
human (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Cancer
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer.
Lymphocytes were fused with NS-1 myeloma cells.
Clinical Data
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer.
Genes Expressed
immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor)
Cellular Products
immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor)
Comments
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer.
Lymphocytes were fused with NS-1 myeloma cells.
Complete Growth Medium
Culture Medium: RPMI medium 1640 with 0.1 mM hypoxanthine, 0.016 mM thymidine,
90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 90%; DMSO, 10%
Isotype
IgM
Name of Depositor
Sloan-Kettering Institute for Cancer Research
Deposited As
human (B cell); mouse (myeloma)
U.S. Patent Number
References

Thomson TM, et al. Human monoclonal antibodies to cell surface antigens. US Patent 4,695,538 dated Sep 22 1987

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