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CF4-C4
CF4-C4
規格:
價格:
編號:B164235
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CF4-C4
商品貨號
B164235
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody; against Lewis a (human blood group antigen),H-2d
Cellular Products
immunoglobulin; monoclonal antibody; against Lewis a (human blood group antigen)
Comments
Mice were immunized with naked Salmonella minnesota to which had been adsorbed the neutral glycolipid fraction from human Type A meconium.
Spleen cells were fused with NS-1 myeloma cells.
The antibody recognizes all Lewis a molecules regardless of the internal linkage of the carbohydrate chain.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
WW Young
Deposited As
mouse (B cell); mouse (myeloma)
References

Young WW Jr., et al. Characterization of monoclonal antibodies specific for the Lewis a human blood group determinant. J. Biol. Chem. 258: 4890-4894, 1983. PubMed: 6833281

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