Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with and neutralizes CDV of the Rockborn, Onderstepoort and Snyder Hill strains.
The antibody may be useful as a reagent for in vitro testing of CDV vaccines.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against canine distemper virus (CDV)
Cellular Products
immunoglobulin; monoclonal antibody; against canine distemper virus (CDV)
Comments
Animals were immunized with canine distemper virus (Rockborn strain).
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with and neutralizes CDV of the Rockborn, Onderstepoort and Snyder Hill strains.
The antibody may be useful as a reagent for in vitro testing of CDV vaccines.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; bovine calf serum, 2.5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
