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當前位置: 首頁 > ATCC代理 > CCRF-SB [CCRF SB] B164219
CCRF-SB [CCRF SB]
CCRF-SB [CCRF SB]
規格:
價格:
編號:B164219
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CCRF-SB [CCRF SB]
商品貨號
B164219
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
B lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 [Cells contain Herpesvirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute lymphoblastic leukemia
Age
11.5 years
Gender
Male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Images
ATCC CCL-120 Cell Micrograph
Derivation

Derived from a buffy coat preparation from a patient with acute lymphoblastic leukemia.

A T cell line (HSB-2, ATCC CCL-120.1) was also obtained from the same buffy coat preparation.

Clinical Data
11.5 years
Caucasian
male
Antigen Expression
HLA A1, A2, B12, B17, Cw2
Comments
The cells are EBNA positive
Complete Growth Medium
RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 105 viable cells/mL. 
Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18
Isoenzymes
G6PD, B
Name of Depositor
GE Foley
Deposited As
Homo sapiens
References

Foley GE et al. M.D. Anderson Hospital and Tumor Inst. Monograph 21, 1967.

Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381

Santra M, et al. Ectopic expression of decorin protein core causes a generalized growth suppression in neoplastic cells of various histogenetic origin and requires endogenous p21, an inhibitor of cyclin-dependent kinases. J. Clin. Invest. 100: 149-157, 1997. PubMed: 9202067

Qian JX, et al. Decreased interleukin-15 from activated cord versus adult peripheral blood mononuclear cells and the effect of interleukin-15 in upregulating antitumor immune activity and cytokine production in cord blood. Blood 90: 3106-3117, 1997. PubMed: 9376592

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