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當(dāng)前位置: 首頁 > ATCC代理 > CCRF-HSB-2 [CCRF HSB-2, HSB-2] B164218
CCRF-HSB-2 [CCRF HSB-2, HSB-2]
CCRF-HSB-2 [CCRF HSB-2, HSB-2]
規(guī)格:
價(jià)格:
編號(hào):B164218
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CCRF-HSB-2 [CCRF HSB-2, HSB-2]
商品貨號(hào)
B164218
Organism
Homo sapiens, human
Tissue
peripheral blood
培養(yǎng)基
Cell Type
T lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute lymphoblastic leukemia
Age
11.5 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Derivation
Derived from the same buffy coat preparation as CCL-120 (CCRF-SB) by serially transplanting into newborn syrian hamsters.
Clinical Data
11.5 years
Caucasian
male
Antigen Expression
HLA A1, A2, B12, B17, Cw2; CD5 (78%), CD7 (96%)
Tumorigenic
Yes, in Syrian hamsters
Effects
Yes, in Syrian hamsters
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL.

A maximum of 2 to 3 x 106 viable cells/mL is obtainable.

Medium Renewal: Every 2 to 3 days

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18,19
Isoenzymes
G6PD, B
Name of Depositor
GE Foley
Deposited As
Homo sapiens
References

Foley GE et al. M.D. Anderson Hospital and Tumor Inst. Monograph 21, 1967.

Adams RA, et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 4872716

Proc. Am. Assoc. Cancer Res. 8: 1, 1967.

Cross References

Nucleotide (GenBank) : NM_004429 Homo sapiens ephrin-B1 (EFNB1), mRNA.

Nucleotide (GenBank) : U09303 Human T cell leukemia LERK-2 (EPLG2) mRNA, complete cds.

Nucleotide (GenBank) : NM_004429 Homo sapiens ephrin-B1 (EFNB1), mRNA.

Nucleotide (GenBank) : U09303 Human T cell leukemia LERK-2 (EPLG2) mRNA, complete cds.

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