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當前位置: 首頁 > ATCC代理 > CCRF-CEM [CCRF CEM] B164217
CCRF-CEM [CCRF CEM]
CCRF-CEM [CCRF CEM]
規格:
價格:
編號:B164217
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CCRF-CEM [CCRF CEM]
商品貨號
B164217
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
T lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute lymphoblastic leukemia
Age
4 years juvenile
Gender
female
Ethnicity
Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 47; range = 41 to 95.
Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted.
Images
Derivation
Human lymphoblasts from peripheral blood of a child with acute leukemia.
Clinical Data
Caucasian
female
4 years juvenile
Antigen Expression
CD3; Homo sapiens, expressed
CD4; Homo sapiens, expressed
CD5; Homo sapiens, expressed
CD7; Homo sapiens, expressed
Genes Expressed
CD3; Homo sapiens, expressed ,CD5; Homo sapiens, expressed ,CD7; Homo sapiens, expressed ,CD4; Homo sapiens, expressed
Tumorigenic
Yes
Effects
Yes, in syrian hamsters
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/mL. Maintain cell density between 2 to 3 X 105 and 1 to 2 X 106 viable cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% DMSO. 
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 10,13
D5S818: 12,13
D7S820: 9,13
THO1: 6,7
TPOX: 8
vWA: 17,19
Isoenzymes
ADA, 1
ES-D, 1
G6PD, B
GLO-I, 1
PEP-D, 1
PGD, C
PGM1, 1
PGM3, 0
Name of Depositor
GE Foley
Deposited As
Homo sapiens
Year of Origin
November, 1964
References

Foley GE, et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965. PubMed: 14278051

Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323

Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381

Uzman BG, et al. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725-1742, 1966. PubMed: 5224274

Adams RA, et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. PubMed: 4295047

Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538

CCRF-CEM is a T lymphoblastoid cell line derived by G.E. Foley, et al. using cells obtained in November, 1964 from peripheral blood buffy coat of a 4-year-old Caucasian female with acute lymphoblastic leukemia.

Cross References

Nucleotide (GenBank) : Z23090 H.sapiens mRNA for 28 kDa heat shock protein.

Nucleotide (GenBank) : AF146431 Homo sapiens cell-line CCRF-CEM sodium/hydrogen exchanger isoform 1 mRNA, complete cds.

Nucleotide (GenBank) : Z12846 HSA08C081 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 08C08, mRNA sequence.

Nucleotide (GenBank) : Z13104 HSA25H071 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 25H07, mRNA sequence.

Nucleotide (GenBank) : Z13398 HSA45A081 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 45A08, mRNA sequence.

Nucleotide (GenBank) : Z13431 HSA48B021 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 48B02, mRNA sequence.

Nucleotide (GenBank) : Z13562 HSA57F051 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 57F05, mRNA sequence.

Nucleotide (GenBank) : Z13677 HSA65D041 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 65D04, mRNA sequence.

Nucleotide (GenBank) : Z13711 HSA68F071 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 68F07, mRNA sequence.

Nucleotide (GenBank) : Z13717 HSA69C041 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 69C04, mRNA sequence.

Nucleotide (GenBank) : Z15500 HSA23D012 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 23D01, mRNA sequence.

Nucleotide (GenBank) : Z15555 HSA27A032 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 27A03, mRNA sequence.

Nucleotide (GenBank) : Z16011 HSA56G072 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 56G07, mRNA sequence.

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