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CCF-STTG1

規格:

價格:

編號:B164215

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

CCF-STTG1

商品貨號

B164215

Organism

Homo sapiens, human

Tissue

brain

培養基

Product Format

frozen

Morphology

astrocytic

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

grade IV, astrocytoma

Age

68 years

Gender

female

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

This human cell line contained large numbers of tetra-, hexa-, and higher-ploid cells (2s populations). The modal (s) cell population which occurred in 32% of cells had the pseudodiploid karyotype, 46,XX,-3,-18,del(7) (q21.12;q22.3), ?t(3q?18q). The rate of 2s cells was 42%. Two marker chromosomes, del(7) (q21.12;q22.3) and ?t(3q?18q), were present in all s metaphases, but ?t(3q?18q) was absent in 2s cells. DMs were found in some s cells whereas they were seen in the majority of 2s cells. HSR chromosomes were not found. In s metaphases, both N3 and N18 had only single copy, and the X chromosome was paired.

Derivation

The CCF-STTG1 cell line was established from a specimen of Grade IV astrocytoma from a 68-year-old Caucasian female by mincing and culturing in RPMI 1640 with 10% FBS.

Clinical Data

68 years

Caucasian

female

Antigen Expression

HLA-DR; Homo sapiens, expressed

Comments

The cells exhibit strong perinuclear acid phosphatase activity and glial fibrillary acidic protein is present in 70% to 80% of the cells.

HLA DR is expressed on 20% to 30% of the cells after 48 hours in culture.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 3 times per week

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 12

D13S317: 11,13

D16S539: 11,12

D5S818: 12,13

D7S820: 10,11

THO1: 7,8

TPOX: 8,11

vWA: 17

Name of Depositor

BP Barna

Deposited As

Homo sapiens

References

. Experimental allergic encephalitis. New York: Liss; 1984.

. . In Vitro 19: 275, 1983.

. . Fed. Proc. 43: 781, 1984.

Barna BP, et al. Enhanced DNA synthesis of human glial cells exposed to human leukocyte products. J. Neuroimmunol. 10: 151-158, 1985. PubMed: 3877740

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