Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
normal
Age
4 years
Gender
male
Applications
The line was derived from normal skin of a child that died of multiple congenital defects.
Derivation
The line was derived from normal skin of a child that died of multiple congenital defects.
Clinical Data
The line was derived from normal skin of a child that died of multiple congenital defects.
male
Comments
The line was derived from normal skin of a child that died of multiple congenital defects.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
