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CCD-1097Sk
CCD-1097Sk
規(guī)格:
價(jià)格:
編號(hào):B164168
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CCD-1097Sk
商品貨號(hào)
B164168
Organism
Homo sapiens, human
Tissue
skin
培養(yǎng)基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Carcinoma
Age
31 years
Gender
female
Ethnicity
Caucasian
Applications
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma.
Derivation
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma.
Clinical Data
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma.
female
Caucasian
31 years
Comments
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma.
Cells from the current passage (1) have experienced about 3 population doublings beyond the biopsy material and are capable of at least 8 additional population doublings.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Passage History
Cells from the current passage (1) have experienced about 3 population doublings beyond the biopsy material and are capable of at least 8 additional population doublings.
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