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CCD-1087Sk
CCD-1087Sk
規(guī)格:
價(jià)格:
編號(hào):B164161
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CCD-1087Sk
商品貨號(hào)
B164161
Organism
Homo sapiens, human
Tissue
skin
培養(yǎng)基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
18 years
Gender
female
Applications
The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty.
Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty.
Clinical Data
The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty.
The patient had interstitial fibrosis and hyalinization consistent with macromastia.
female
Comments
The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty.
The patient had interstitial fibrosis and hyalinization consistent with macromastia.
The cells senesce after approximately 43 population doublings.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
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