Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
normal
Age
newborn
Gender
male
Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was established from skin taken from normal foreskin.
Clinical Data
male
newborn
Comments
Cells senesce after approximately 36 population doublings.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Population Doubling Capacity
Cells senesce after approximately 36 population doublings.
